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Vimentin expression was again detected in invading trophoblasts. Serum-free culture of villous stroma resulted in loss of villous structure, epithelial disintegration and EMT. The dynamic downregulation of E-cadherin expression that occurs in the villous stroma during invasion, and the induction of vimentin expression in the villous and decidual stromal cells suggest that they have a role in the EMT process. Further analysis of the EMT process is needed to characterize factors that affect invasion.A review of experimental cholesteatoma research. Cholesteatoma is a common otological disorder that has traditionally been associated with persistent infection. It is a multifactorial disorder that is associated with genetic, environmental, and behavioral factors. Experimental cholesteatoma research has provided valuable information toward gaining a better understanding of the pathophysiology of cholesteatoma. 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